Proteomic profiling of lymphedema development in mouse model.
Identifieur interne : 000C23 ( Main/Exploration ); précédent : 000C22; suivant : 000C24Proteomic profiling of lymphedema development in mouse model.
Auteurs : Joomin Lee [Corée du Sud] ; Haeun Song [Corée du Sud] ; Kangsan Roh [Corée du Sud] ; Sungrae Cho [Corée du Sud] ; Sukchan Lee [Corée du Sud] ; Chang-Hwan Yeom [Corée du Sud] ; Seyeon Park [Corée du Sud]Source :
- Cell biochemistry and function [ 1099-0844 ] ; 2016.
Descripteurs français
- KwdFr :
- Animaux, Lymphoedème (anatomopathologie), Lymphoedème (métabolisme), Membre pelvien (anatomopathologie), Modèles animaux de maladie humaine, Mâle, Protéome (métabolisme), Protéomique (), Reproductibilité des résultats, Souris de lignée ICR, Technique de Western, Électrophorèse bidimensionnelle sur gel.
- MESH :
- anatomopathologie : Lymphoedème, Membre pelvien.
- métabolisme : Lymphoedème, Protéome.
- Animaux, Modèles animaux de maladie humaine, Mâle, Protéomique, Reproductibilité des résultats, Souris de lignée ICR, Technique de Western, Électrophorèse bidimensionnelle sur gel.
English descriptors
- KwdEn :
- MESH :
- chemical , metabolism : Proteome.
- metabolism : Lymphedema.
- methods : Proteomics.
- pathology : Hindlimb, Lymphedema.
- Animals, Blotting, Western, Disease Models, Animal, Electrophoresis, Gel, Two-Dimensional, Male, Mice, Inbred ICR, Reproducibility of Results.
Abstract
The lymphatic vascular system plays an important role in tissue fluid homeostasis. Lymphedema is a chronic, progressive, and incurable condition that leads to lymphatic fluid retention; it may be primary (heritable) or secondary (acquired) in nature. Although there is a growing understanding of lymphedema, methods for the prevention and treatment of lymphedema are still limited. In this study, we investigated differential protein expressions in sham-operated and lymphedema-operated mice for 3 days, using two-dimensional gel electrophoresis (2-DE) and mass spectrometry analysis. Male improved methodology for culturing noninbred (ICR) mice developed lymphedema in the right hindlimb. Twenty functional proteins were found to be differentially expressed between lymphedema induced-right leg tissue and normal left leg tissue. Out of these proteins, the protein levels of apolipoprotein A-1 preprotein, alpha-actinin-3, mCG21744, parkinson disease, serum amyloid P-component precursor, annexin A8, mKIAA0098 protein, and fibrinogen beta chain precursor were differentially upregulated in the lymphedema mice compared with the sham-operated group. Western blotting analysis was used to validate the proteomics results. Our results showing differential up-regulation of serum amyloid P-component precursor, parkinson disease, and apolipoprotein A-1 preprotein in lymphedema model over sham-operated model suggest important insights into pathophysiological target for lymphedema. Copyright © 2016 John Wiley & Sons, Ltd.
DOI: 10.1002/cbf.3192
PubMed: 27151289
Affiliations:
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Kangsan" sort="Roh, Kangsan" uniqKey="Roh K" first="Kangsan" last="Roh">Kangsan Roh</name><affiliation wicri:level="1"><nlm:affiliation>Department of Genetic Engineering, Sungkyunkwan University, Suwon, Republic of Korea.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Department of Genetic Engineering, Sungkyunkwan University, Suwon</wicri:regionArea><wicri:noRegion>Suwon</wicri:noRegion></affiliation></author><author><name sortKey="Cho, Sungrae" sort="Cho, Sungrae" uniqKey="Cho S" first="Sungrae" last="Cho">Sungrae Cho</name><affiliation wicri:level="1"><nlm:affiliation>Department of Genetic Engineering, Sungkyunkwan University, Suwon, Republic of Korea.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Department of Genetic Engineering, Sungkyunkwan University, Suwon</wicri:regionArea><wicri:noRegion>Suwon</wicri:noRegion></affiliation></author><author><name sortKey="Lee, Sukchan" sort="Lee, Sukchan" uniqKey="Lee S" 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uniqKey="Park S" first="Seyeon" last="Park">Seyeon Park</name><affiliation wicri:level="3"><nlm:affiliation>Department of Applied Chemistry, Dongduk Women's University, Seoul, Republic of Korea.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Department of Applied Chemistry, Dongduk Women's University, Seoul</wicri:regionArea><placeName><settlement type="city">Séoul</settlement><region type="capital">Région capitale de Séoul</region></placeName></affiliation></author></analytic><series><title level="j">Cell biochemistry and function</title><idno type="eISSN">1099-0844</idno><imprint><date when="2016" type="published">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Blotting, Western</term><term>Disease Models, Animal</term><term>Electrophoresis, Gel, Two-Dimensional</term><term>Hindlimb (pathology)</term><term>Lymphedema (metabolism)</term><term>Lymphedema (pathology)</term><term>Male</term><term>Mice, Inbred ICR</term><term>Proteome (metabolism)</term><term>Proteomics (methods)</term><term>Reproducibility of Results</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Lymphoedème (anatomopathologie)</term><term>Lymphoedème (métabolisme)</term><term>Membre pelvien (anatomopathologie)</term><term>Modèles animaux de maladie humaine</term><term>Mâle</term><term>Protéome (métabolisme)</term><term>Protéomique ()</term><term>Reproductibilité des résultats</term><term>Souris de lignée ICR</term><term>Technique de Western</term><term>Électrophorèse bidimensionnelle sur gel</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Proteome</term></keywords><keywords scheme="MESH" qualifier="anatomopathologie" xml:lang="fr"><term>Lymphoedème</term><term>Membre pelvien</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Lymphedema</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Proteomics</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Lymphoedème</term><term>Protéome</term></keywords><keywords scheme="MESH" qualifier="pathology" xml:lang="en"><term>Hindlimb</term><term>Lymphedema</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Blotting, Western</term><term>Disease Models, Animal</term><term>Electrophoresis, Gel, Two-Dimensional</term><term>Male</term><term>Mice, Inbred ICR</term><term>Reproducibility of Results</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Modèles animaux de maladie humaine</term><term>Mâle</term><term>Protéomique</term><term>Reproductibilité des résultats</term><term>Souris de lignée ICR</term><term>Technique de Western</term><term>Électrophorèse bidimensionnelle sur gel</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The lymphatic vascular system plays an important role in tissue fluid homeostasis. Lymphedema is a chronic, progressive, and incurable condition that leads to lymphatic fluid retention; it may be primary (heritable) or secondary (acquired) in nature. Although there is a growing understanding of lymphedema, methods for the prevention and treatment of lymphedema are still limited. In this study, we investigated differential protein expressions in sham-operated and lymphedema-operated mice for 3 days, using two-dimensional gel electrophoresis (2-DE) and mass spectrometry analysis. Male improved methodology for culturing noninbred (ICR) mice developed lymphedema in the right hindlimb. Twenty functional proteins were found to be differentially expressed between lymphedema induced-right leg tissue and normal left leg tissue. Out of these proteins, the protein levels of apolipoprotein A-1 preprotein, alpha-actinin-3, mCG21744, parkinson disease, serum amyloid P-component precursor, annexin A8, mKIAA0098 protein, and fibrinogen beta chain precursor were differentially upregulated in the lymphedema mice compared with the sham-operated group. Western blotting analysis was used to validate the proteomics results. Our results showing differential up-regulation of serum amyloid P-component precursor, parkinson disease, and apolipoprotein A-1 preprotein in lymphedema model over sham-operated model suggest important insights into pathophysiological target for lymphedema. Copyright © 2016 John Wiley & Sons, Ltd.</div></front></TEI><affiliations><list><country><li>Corée du Sud</li></country><region><li>Région capitale de Séoul</li></region><settlement><li>Séoul</li></settlement></list><tree><country name="Corée du Sud"><noRegion><name sortKey="Lee, Joomin" sort="Lee, Joomin" uniqKey="Lee J" first="Joomin" last="Lee">Joomin Lee</name></noRegion><name sortKey="Cho, Sungrae" sort="Cho, Sungrae" uniqKey="Cho S" first="Sungrae" last="Cho">Sungrae Cho</name><name sortKey="Lee, Sukchan" sort="Lee, Sukchan" uniqKey="Lee S" first="Sukchan" last="Lee">Sukchan Lee</name><name sortKey="Park, Seyeon" sort="Park, Seyeon" uniqKey="Park S" first="Seyeon" last="Park">Seyeon Park</name><name sortKey="Roh, Kangsan" sort="Roh, Kangsan" uniqKey="Roh K" first="Kangsan" last="Roh">Kangsan Roh</name><name sortKey="Song, Haeun" sort="Song, Haeun" uniqKey="Song H" first="Haeun" last="Song">Haeun Song</name><name sortKey="Yeom, Chang Hwan" sort="Yeom, Chang Hwan" uniqKey="Yeom C" first="Chang-Hwan" last="Yeom">Chang-Hwan Yeom</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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